Book Chapter

Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells upon EnteropathogenicEscherichia coli Infection (link, full text)
Victor Yashunsky,  Benjamin Aroeti

Book Title
Label-Free Biosensor Methods in Drug Discovery
Edited by Ye Fang
Pages
pp 353-371
DOI
10.1007/978-1-4939-2617-6_20

Abstract
Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.