SINICA TALK

Victor Yashunsky seminar on Live cell measurements with IR-SPR during a visit in Prof. Keng hui Lin lab.
https://sites.google.com/site/psroceletter/home/021-030/2014-10-31#speeches-3

Book Chapter

Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells upon EnteropathogenicEscherichia coli Infection (link, full text)
Victor Yashunsky,  Benjamin Aroeti

Book Title
Label-Free Biosensor Methods in Drug Discovery
Edited by Ye Fang
Pages
pp 353-371
DOI
10.1007/978-1-4939-2617-6_20

Abstract
Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

New JBO paper by A. Zilbershtein and A. Bein

Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control

Abstract: We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular 

Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor.