New JBO paper by A. Zilbershtein and A. Bein

Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control

Abstract: We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular 

Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor.

The 2nd Conference of the Israel Society for Biotechnology Engineering (ISBE)

Two talks from our group will be delivered at the 2nd Conference of the Israel Society for Biotechnology Engineering at Biosensor session

December 1, 2013, Dan Hotel, Tel-Aviv

14:45 REAL-TIME SENSING OF HOST-PATHOGEN INTERACTIONS BY INFRARED SURFACE PLASMON SPECTROSCOPY

Benjamin Aroeti, Victor Yashunsky, Leorah Kharilker, Efrat Zlotkin-Rivkin, David Rund, Naomi Melamed-Book, Eitan Zahavi, Eran Perlson, Silvana Mercone, Michael Golosovsky, Dan Davidov

15:55 THE USE OF SURFACE PLASMON-BASED INFRARED SPECTROSCOPY TO MONITOR INTERCELLULAR JUNCTIONS IN NORMAL AND INFLAMED INTESTINAL CELLS

Alexander Zilbershtein, Amir Bein, Michael Golosovsky, Dan Davidov, Betty Schwartz

Hot from the oven: New PLoS ONE paper on cell-bacteria interaction monitored by IR-SPR

Yashunsky V, Kharilker L, et al, Real-Time Sensing of Enteropathogenic E. coli-Induced Effects on Epithelial Host Cell Height, Cell-Substrate Interactions, and Endocytic Processes by Infrared Surface Plasmon Spectroscopy. PLoS ONE 8(10): e78431 (2013). doi:10.1371/journal.pone.0078431